TABLE 2.

Primers used in this study

Primer	Description	Sequence (5′ to 3′)
idhfor	N-terminal forward for idh	AGCTGCGGCTCGACCAGGAT
idhrev	C-terminal reverse for idh	AGCTCCCAGTTCGGCCCGTA
idhxf	N-terminal forward for idh with added XbaI site	TCTAGAAGCTGCGGCTCGACCAGGAT
idhxr	C-terminal reverse for idh with added XbaI site	AGCTCCCAGTTCGGCCCGTATCTAGA
strfor	N-terminal forward for streptomycin	ACATTTGTACGGCTCCGCA
strrev	C-terminal reverse for streptomycin	TTAAGGTTTCATTTAGCGCCTCAAATAGA
idhfor2	N-terminal forward for genomic DNA flanking idh	AATCGGCTGAGCGCAAAATT
strrev2	C-terminal reverse for streptomycin	ATGCGGATCAGTGAGGGTTT
idhhindf	N-terminal forward for idh with added HindIII site	TAGGCACCCCAGGCTTTACACTTTATGCTTCC
idhhindr	C-terminal reverse for kanamycin with added HindIII site	CCCAAGCTTGGGTGAGCAAAAACAGGACAAAA