TABLE 3.
Enzyme activities in cell extracts of B. japonicum USDA110, 5051, and 5051(pRS7) cultured on arabinose
| Enzyme | Enzyme activity (nmol/min/mg protein) of straina:
|
||
|---|---|---|---|
| USDA110 | 5051 | 5051(pRS7) | |
| Isocitrate dehydrogenase | 290 ± 61 | 0.4 ± 0.3 | 451 ± 267 |
| Citrate synthase | 429 ± 33 | 526 ± 37 | 625 ± 134 |
| α-Ketoglutarate dehydrogenase | 245 ± 33 | 109 ± 14 | 277 ± 89 |
| Malate dehydrogenase | 2,354 ± 204 | 2,184 ± 187 | 2,225 ± 332 |
| Hydroxybutyrate dehydrogenase | 84 ± 9 | 76 ± 11 | 84 ± 12 |
| Glyceraldehyde-3-phosphate dehydrogenase | 1,230 ± 57 | 1,303 ± 78 | 1,202 ± 111 |
| Isocitrate lyase | 0.7 ± 0.3 | 0.6 ± 0.4 | 0.7 ± 0.4 |
a
The values shown are the means ± standard deviations for three different cell extracts, each measured at two different enzyme levels.