FIG. 2.

Adenine DNA glycosylase activities of HP0142. DNA glycosylase activities were determined using a 34-mer oligonucleotide carrying an A/8-oxoG pair at position 16. The strand labeled is the one carrying the A. The products of the reaction (P) were separated from the substrates (S) by denaturing gel electrophoresis. (A) Activities of the indicated amounts of cell extracts from E. coli wild-type (w.t.) (lanes 2 and 3) or mutY strains (lanes 4 to 7) carrying the vector (lanes 4 and 5) or the HP0142-expressing plasmid (lanes 6 and 7). (B) Activities of the HP0142 purified protein. Reactions were stopped with (lanes 2 and 4) or without (lane 3) NaOH. C, control.