FIG. 5.
Interactions between His-PcrASpn and RepC. The physical interaction between PcrASpn and RepC proteins was analyzed by performing a pull-down assay as described in Materials and Methods. The fractions eluted from amylose resin columns (containing [+] or lacking [−] bound MBP-RepC) were probed with either anti-MBP or anti-His6 monoclonal antibodies (Ab). In a control experiment in which MBP-RepC was not adsorbed to the amylose columns (lane 3), 1% BSA was used instead. In lane His-PcrASpn load, 0.2 μg of purified pneumococcal protein was loaded directly onto the gel.