Skip to main content
. 2006 Aug 25;188(21):7416–7425. doi: 10.1128/JB.01010-06

FIG. 6.

FIG. 6.

DNA binding and helicase activities of PcrASpn. (A) PcrASpn shows specific binding to DNA substrates containing a hairpin structure at their 3′ or 5′ end. EMSA were carried out by incubating His-PcrASpn at different molar ratios with four different 5′ 32P-labeled probes. The DNA-protein complexes were resolved on 6% polyacrylamide gels. (B) PcrASpn showed bipolar 5′→3′ and 3′→5′ helicase activities. His-PcrASpn was incubated with 32P-labeled partially duplex substrates containing either 3′ or 5′ oligo(dT) tails (substrates A and B) or with structured substrates containing 5′ or 3′ ssDNA regions (substrates C and D). The reaction products were resolved by native PAGE.