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In vitro replication of plasmid pT181. In vitro replication was carried out using the RepC protein and cell extracts from either wild-type S. aureus (wt ext) or the pcrA3 mutant of S. aureus (PcrA- ext) and different amounts of PcrA helicases. The positions of supercoiled pT181cop608 DNA (SC), linear DNA (L), open circular DNA (OC), and replication intermediates (RI) are indicated.
