TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli M15 | K-12 derivative | Qiagen |
| E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS mcrBC) φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| S. pneumoniae R6 | Wild type | 27 |
| S. aureus RN4220 | Restriction-deficient mutant of strain 8325-4 | 25 |
| S. aureus RN4220 pcrA3 | pcrA3 mutant | 17 |
| Plasmids | ||
| pT181-cop608 | Copy mutant of pT181 | 38 |
| pCR2.1 | pUC Plac lacZα pT7 neo bla f1-ori | Invitrogen |
| pCR2.1C | pCR2.1 cat | This study |
| pCR2.11 | pCR2.1 Δ3′pcrA | This study |
| pCR2.12 | pCR2.1 Δ5′pcrA | This study |
| pCR2.13 | pCR2.1 Δ5′3′pcrA | This study |
| pCR2.1C1 | pCR2.1 cat Δ3′pcrA | This study |
| pCR2.1C2 | pCR2.1 cat Δ5′pcrA | This study |
| pCR2.1C3 | pCR2.1 cat Δ5′3′pcrA | This study |
| pCR2.1PN | pCR2.1 pcrASpn | This study |
| pQE30 | QIAGEN | |
| pQE30PA | pQE30 His-pcrASau | 9 |
| pQE30PN | pQE30 His-pcrASpn | This study |
| pSK1377 | MBP-wild-type RepC | 49 |