TABLE 1.
E. coli strains
| Straina | Relevant genotype | Reference or source |
|---|---|---|
| AB1157 | rec+sbc+b | 6 |
| JC5519 | recB21 recC22 | 6 |
| JC7623 | recB21 recC22 sbcB15 sbcC201 | 23 |
| JC8260 | recB21 recC22 xonA2 hisG+ | 22 |
| JJC260 | sbcD300::kan | B. Michel |
| JJC889 | ΔsbcB::cam | 9 |
| N2364 | sbcC201 phoR79::Tn10 | 25 |
| BW6156 | Hfr proAB+ (PO3 of P4X) | 42 |
| K797 | phoR79::Tn10 | CGSC 6456c |
| KL742 | hisG+thyA748::Tn10 | CGSC 6212c |
| V330 | Δ(recC-argA)234 | 2 |
| WA576 | recF400::Tn5 | B. Micheld |
| SWM1003 | ΔrecQ::kan | 25 |
| LMM981 | recB21 recC22 sbcB15 sbcC201 hisG+ | P1.KL742 × JC7623 to His+ |
| LMM1124 | recB21 recC22 ΔsbcB::cam | P1.JJC889 × JC5519 to Cmr |
| LMM1128 | recB21 recC22 sbcB15 hisG+ | P1.LMM981 × LMM1124 to His+ Cms UVr |
| LMM1298 | recB21 recC22 sbcC201 phoR79::Tn10 | P1.N2364 × JC5519 to Tcr λpals |
| LMM1329 | recB21 recC22 ΔsbcB::cam sbcC201 phoR79::Tn10 | P1.JJC889 × LMM1298 to Cmr UVr |
| LMM1330 | recB21 recC22 sbcB15 hisG+sbcC201 phoR79::Tn10 | P1.LMM981 × LMM1329 to His+ Cms |
| LMM1331 | recB21 recC22 ΔsbcB::cam sbcC201 phoR79::Tn10 recF400::Tn5 | P1.WA576 × LMM1329 to Kmr UVs |
| LMM1332 | recB21 recC22 sbcB15 hisG+sbcC201 phoR79::Tn10 recF400::Tn5 | P1.WA576 × LMM1330 to Kmr UVs |
| LMM1362 | recB21 recC22 ΔsbcB::cam sbcC201 phoR79::Tn10 ΔrecQ::kan | P1.SWM1003 × LMM1329 to Kmr UVs |
| LMM1363 | recB21 recC22 sbcB15 hisG+sbcC201 phoR79::Tn10 ΔrecQ::kan | P1.SWM1003 × LMM1330 to Kmr UVs |
| LMM1745 | recB21 recC22 xonA2 hisG+sbcC201 phoR79::Tn10 | P1.JC8260 × LMM1329 to His+ Cms |
| LMM1746 | recB21 recC22 xonA2 hisG+sbcC201 phoR79::Tn10 recF400::Tn5 | P1.WA576 × LMM1745 to Kmr UVs |
| LMM1747 | recB21 recC22 xonA2 hisG+sbcC201 phoR79::Tn10 ΔrecQ::kan | P1.SWM1003 × LMM1745 to Kmr UVs |
| LMM1748 | recB21 recC22 xonA2 hisG+sbcD300::kan phoR+ | P1.JJC260 × LMM1745 to Kmr Tcs |
| LMM1749 | recB21 recC22 xonA2 hisG+sbcD+phoR79::Tn10 | P1.K797 × LMM1748 to Tcr Kms UVs λpalr |
| LMM1721 | thyA748::Tn10 | P1.KL742 × AB1157 to Tcrthy |
| LMM1724 | Δ(recC-argA)234 thyA+ | P1.V330 × LMM1721 to thy+ UVs T42s |
| LMM1725 | Δ(recC-argA)234 thyA+ ΔsbcB::cam | P1.JJC889 × LMM1724 to Cmr |
| LMM1726 | Δ(recC-argA)234 thyA+sbcB15 hisG+ | P1.LMM981 × LMM1725 to His+ UVr |
| LMM1732 | Δ(recC-argA)234 thyA+ ΔsbcB::cam sbcC201 phoR79::Tn10 | P1.N2364 × LMM1725 to Tcr UVr λpals |
| LMM1733 | Δ(recC-argA)234 thyA+sbcB15 hisG+sbcC201 phoR79::Tn10 | P1.LMM981 × LMM1732 to His+ Cms |
a
All strains except BW6156, K797, KL742, and V330 are derivatives of AB1157.
b
Other markers are F− thr-1 ara-14 leuB6 Δ(gpt-proA)62 lacY1 tsx-33 supE44 galK2 λ− rac− hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr′.
c
Strain supplied by M. Berlyn of the Escherichia coli Genetic Stock Center.
d
Strain originated from the laboratory of W. Wackernagel.