TABLE 2.

Conjugational recombination with different sbcB recipient strains

Recipient strain	Relevant genotype	Relative viabilitya	Relative yield of recombinantsb
AB1157	Wild type	1	1
JC5519	recB21 recC22	0.27 ± 0.022	0.008 ± 0.0014
LMM1124	recB21 recC22 ΔsbcB	0.25 ± 0.04	0.015 ± 0.0016
LMM1128	recB21 recC22 sbcB15	0.18 ± 0.042	0.18 ± 0.054
LMM1298	recB21 recC22 sbcC201	0.33 ± 0.037	0.005 ± 0.001
LMM1329	recB21 recC22 ΔsbcB sbcC201	0.39 ± 0.088	0.16 ± 0.038
LMM1330	recB21 recC22 sbcB15 sbcC201	0.75 ± 0.125	1.4 ± 0.24
LMM1331	recB21 recC22 ΔsbcB sbcC201 recF400	0.08 ± 0.022	0.0022 ± 0.0013
LMM1332	recB21 recC22 sbcB15 sbcC201 recF400	0.12 ± 0.045	0.1 ± 0.02
LMM1362	recB21 recC22 ΔsbcB sbcC201 ΔrecQ	0.36 ± 0.042	0.007 ± 0.002
LMM1363	recB21 recC22 sbcB15 sbcC201 ΔrecQ	0.52 ± 0.086	0.087 ± 0.014
LMM1749	recB21 recC22 xonA2	0.21 ± 0.026	0.017 ± 0.004
LMM1745	recB21 recC22 xonA2 sbcC201	0.41 ± 0.012	0.14 ± 0.046
LMM1746	recB21 recC22 xonA2 sbcC201 recF400	0.15 ± 0.061	0.0017 ± 0.0005
LMM1747	recB21 recC22 xonA2 sbcC201 ΔrecQ::kan	0.40 ± 0.056	0.0075 ± 0.0007
LMM1724	ΔrecBCD	0.29 ± 0.032	0.0046 ± 0.0006
LMM1725	ΔrecBCD ΔsbcB	0.23 ± 0.061	0.0075 ± 0.0039
LMM1726	ΔrecBCD sbcB15	0.19 ± 0.04	0.16 ± 0.026
LMM1732	ΔrecBCD ΔsbcB sbcC201	0.42 ± 0.11	0.13 ± 0.026
LMM1733	ΔrecBCD sbcB15 sbcC201	0.73 ± 0.035	1.1 ± 0.10

^aViability was determined for cultures grown to an OD₆₀₀ of 0.3 and is expressed relative to the number of CFU per milliliter in cultures of control recipient strain AB1157, which averaged 1.5 × 10⁸ CFU/ml. The values are averages ± standard deviations of results from at least three independent experiments.

^bThe yields of recombinants are relative to the yield of control strain AB1157 and were corrected for any deficiency in the viability of the recipient strain. The average yield for control strain AB1157 was 4.5 × 10⁵ CFU per ml of the mating mixture. The values are averages ± standard deviations of results from at least three independent experiments.