TABLE 1.
Distribution of 329 ORFs that were differentially expressed in at least one mutant compared to in the wild-type strain as of 14 h of nitrogen deprivation, in terms of cluster analysis and functional categorya
| Functional category | No. of genes in cluster:
|
Total no. of genes | ||||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | ||
| Biosynthesis of cofactors, prosthetic groups, and carriers | 3 | 1 | 1 | 5 | ||||
| Cell envelope | 14 | 1 | 1 | 1 | 1 | 18 | ||
| Energy metabolism | 6 | 1 | 7 | |||||
| Glycosyl transferases | 20 | 1 | 3 | 24 | ||||
| Heterocyst differentiation | 3 | 5 | 8 | 2 | 1 | 19 | ||
| Hypothetical and unknown | 81 | 9 | 5 | 7 | 34 | 2 | 10 | 148 |
| Nitrogen fixation and metabolism | 22 | 22 | ||||||
| Other | 23 | 14 | 1 | 38 | ||||
| Photosynthesis and respiration | 4 | 5 | 1 | 10 | ||||
| Regulatory functions | 6 | 1 | 5 | 1 | 13 | |||
| Transcription and translation | 2 | 1 | 1 | 4 | ||||
| Transport and binding proteins | 3 | 1 | 4 | 1 | 9 | |||
| Transposase | 2 | 2 | 8 | 12 | ||||
| Total | 189 | 18 | 13 | 9 | 70 | 7 | 23 | 329 |
a
Cluster analysis was carried out as described in Materials and Methods. The functional classification is based on that of the Kazusa DNA Research Institute (http://www.kazusa.or.jp/cyanobase/Anabaena/) with minor modifications as described in the text.