TABLE 2.
Regulatory genes and genes related to transcription and translation that are, at 14 h of nitrogen deprivation, differentially expressed in at least one mutant relative to in wild-type Anabaena sp. strain PCC 7120
| ORF | Gene | Description of product | Cluster | Regulation in mutant:
|
|||
|---|---|---|---|---|---|---|---|
| henR | hepS | hepN | hepK | ||||
| all0648 | Probable anti-sigma factor antagonist | 1 | Down | Down | |||
| all2164 | Two-component system, regulatory protein | 1 | Down | Down | |||
| all2165 | Two-component response regulator with PATAN domain | 1 | Down | Down | |||
| all3447 | Unknown protein with a DNA-binding HTHdomain | 1 | Down | Down | |||
| alr5251 | Two-component response regulator | 1 | Down | Down | |||
| alr1381 | prcA | Ca2+-dependent protease | 1 | (Down)a | Down | ||
| alr3170 | Hypothetical protein containing PAS, PAC, and GGDEF domains | 1 | Down | Down | Down | ||
| all4927 | Two-component response regulator | 1 | Down | ||||
| all2368 | Translation initiation factor 3 | 2 | Down | ||||
| all2883 | Two-component sensor histidine kinase | 4 | Down | ||||
| all0521 | patA | Two-component response regulator with PATAN domain | 5 | Up | Up | Up | |
| asl0125 | Transcriptional regulator | 5 | Up | Up | |||
| all1072 | Two-component response regulator with PATAN domain | 5 | Up | Up | |||
| all1173 | DNA-binding protein, starvation inducible | 5 | Up | Up | |||
| alr1869 | Serine/threonine kinase | 5 | Up | Up | |||
| asl2551 | Transcriptional regulator | 5 | (Up)b | Up | |||
| all2760c | hepS | Serine/threonine kinase | 5 | Up | |||
| all0438c | Serine/threonine kinase with WD-40 repeats | 6 | Up | ||||
| all4496d | hepK | Two-component system sensory histidine kinase | 6 | Up | |||
| all0844 | Peptidyl-tRNA hydrolase | 7 | Up | ||||
a
Down-regulated more than twofold, a ratio not statistically significant because of a large variation among experimental replicates. alr1381 is not a Fox gene (47).
b
Up-regulated 1.98-fold in the hepN mutant, with a PPDE (<P) value of 0.989.
c
Up-regulated at 8 h in wild-type Anabaena sp. strain PCC 7120 (13).
d
Up-regulated at 3 and 8 h in wild-type Anabaena sp. strain PCC 7120 (13).