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Analysis by flow cytometry of binding of the anti-BclA MAb EF12 to wild-type and ΔexsY spores of B. anthracis. Spores of the wild-type Sterne strain (WT) produced in liquid medium (A) and spores of strain CLT325 (ΔexsY) produced on solid (B) or in liquid (C) medium were treated with fluorescently labeled EF12 (unfilled histograms with thick outlines) or an equivalently labeled isotype control MAb (gray histograms).
