Alignment of nrf promoter sequences from different enteric bacteria. The figure shows the sequence of the E. coli K-12 pnrf53 fragment from positions −209 to +10 (NC00913), aligned with the nrf promoter regions from enterohemorrhagic E. coli (EHEC) (NC002695), uropathogenic E. coli (UPEC) (NC004431), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), Shigella flexneri (SFX) (NC004741), and S. enterica serovar Typhimurium (STM) (NC003197). The location of the transcription start site for pnrf53 is indicated in lowercase. The locations of FNR, CRP, and NarL/NarP binding sites are represented by inverted arrows, while IHF and Fis binding sites are depicted by boxes. The insertion of sequences within the upstream promoter region of the S. enterica serovar Typhimurium promoter is indicated. Differences between the pnrf53 fragment and other promoters are highlighted by black boxes. The position at which upstream sequences were deleted from the S. enterica serovar Typhimurium promoter to generate the pnrf53/Δ87 STM promoter fragment is indicated by Δ87.