FIG. 4.

In vitro activities of σ²⁸-dependent promoters. (a) Promoter sequences inserted into the test vector. (b) Gel analysis of the in vitro transcription products using RNAP-σ²⁸_Ct (upper panel) or RNAP-σ²⁸_EC (lower panel). Supercoiled DNA templates used in this experiment are indicated on the top of each lane. The RNAP holoenzymes used are indicated on the left. Note that all transcripts were generated from the same starting amount of DNA template and run in parallel on the same gel. The ratios between the amounts of the test promoter and control promoter transcripts (P_test/P_fliC) are indicated and were used to normalize the data.