TABLE 1.
Names and descriptions of plasmids
| Plasmid | Description | Source |
|---|---|---|
| pLC3 | A low-copy-number expression vector; a recombinant of pBAD24 and pACYC184; the origin of replication of p15A, Tcr | 49 |
| pLF28 | pLC3 carrying chlamydial rpsD | 49 |
| pLE28 | pLC3 carrying E. coli fliA | 49 |
| pS28H | A derivative of pBAD24; expression vector with an N-terminal His6 tag; carries 5′ chlamydial rpsD; Apr | 49 |
| pES28H | A derivative of pBAD24; expression vector with an N-terminal His6 tag; carries E. coli fliA; Apr | 49 |
| pRVB | A recombinant of pRV1 and pBR322; ColE1; Apr | This study |
| pCG | A promoter probe vector containing promoterless cat::gfp; the origin of replication of ColE1; Apr | This study |
| pPgroE::cat-gfp | pCG with the promoter region of chlamydial groE (PgroE) | This study |
| pPgroEEc::cat-gfp | pCG with the promoter region of E. coli groE (PgroEEc) | This study |
| pPflgK::cat-gfp | pCG with the promoter region of E. coli flgK (PflgK) | This study |
| pPhctB::cat-gfp | pCG with the promoter region of chlamydial hctB (PhctB) | This study |
| pPtar::cat-gfp | pCG with the promoter region of E. coli tar (Ptar) | This study |
| pPycgR::cat-gfp | pCG with the promoter region of E. coli ycgR (PycgR) | This study |
| pMT504 | A cloning vector containing a promoterless guanine (G)-less cassette and a chalmydial rRNA P1-controlled G-less cassette; Apr | 53 |
| pGLC | A cloning vector containing a promoterless guanine (G)-less cassette and an E. coli fliC promoter-controlled G-less cassette; Apr | 49 |
| pGPgroEEc | pGLC with the promoter region of E. coli groE (PfliC and PgroEEc) | This study |
| pGPgroE | pGLC with the promoter region of chlamydial groE from serovar F | 49 |
| pGPflgK | pGLC with the promoter region of E. coli flgK (PfliC and PflgK) | This study |
| pGPtar | pGLC with the promoter region of E. coli tar (PfliC and Ptar) | This study |
| pGPycgR | pGLC with the promoter region of E. coli ycgR (PfliC and PycgR) | This study |
| pGPhctB | pGLC with the promoter region of chlamydial hctB from serovar F | This study |
| pGPhctBΔUP | A derivate of pGPhctB deleting the upstream sequence of the PhctB−35 region | This study |
| pGPhctB-2 | A derivate of pGPhctB fusing a DNA sequence upstream of Ptar to the core promoter of PhctB | This study |
| pGPtar+ | A derivate of pGPtar fusing a DNA sequence upstream of PhctB to the core promoter of Ptar | This study |
| pGPlacUV5 | pGLC with the promoter region of E. coli PlacUV5 | This study |
| pGPlacUV5+ | A derivate of pGPlacUV5 containing the upstream DNA −65 to −38 sequence fused to core the promoter of PlacUV5 | This study |
| pGS9 | A derivate of pGPhctB containing 9 bp between the −35 and −10 regions | This study |
| pGS10 | A derivate of pGPhctB containing 10 bp between the −35 and −10 regions | This study |
| pGS11 | A derivate of pGPhctB containing 11 bp between the −35 and −10 regions | This study |
| pGS13 | A derivate of pGPhctB containing 13 bp between the −35 and −10 regions | This study |
| pGS14 | A derivate of pGPhctB containing 14 bp between the −35 and −10 regions | This study |