TABLE 1.
Primers used in this study
| Primera | Primer sequence (5′-3′) |
|---|---|
| cat1 | CAGGGTCGTTAAATAGCCGC |
| arb1 | GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT |
| cat2 | CCGTTGCTTCTCAAATGCC |
| arb2 | GGCCACGCGTCGACTAGTAC |
| yihO_A | AGCCCAAGCTTAAAAAACCCATCCATGA |
| yihO_B | TCTAATCATGATCCGCGTTAATTAATTAACGCCACTCACTTTACTTTACCCGTTAAAACA |
| yihO_C | AGTAAAGTGAGTGGCGTTAATTAATTAACGCGGATCATGATTAGACATAATCGCCTCTCA |
| yihO_D | TCGGCGGGCTGTTAATATGG |
| yihQE_A | ACCGTAAGCTTTTACGCCTGCGTTGCGC |
| yihQE_B | AATTCTCTACCACAACGTTAATTAATTAACCTGCAAAACGACGACCTGCACCATAACCGT |
| yihQE_C | GTCGTCGTTTTGCAGGTTAATTAATTAACGTTGTGGTAGAGAATTCATTGTAATCTCCG |
| yihQE_D | TCGGTTAGATGCCGCCAGTG |
| agfD_A | GACGAATTCGTGTGTTATGCCGCCATGGG |
| agfD_B | GGACTGCAGTAAACATGATG |
| agfD_C | GCCCTGCAGCAAACGATAATCTCAGGCGG |
| agfD_D | GCCAAGCTTTGTCCGTGACGTTGAGCTGG |
| ΔPyih_A | AGGGGAAGCTTGCTCTACTTGCTCCGCC |
| ΔPyih_B | CGAAATGTTTAAAAAAAAAAAGCTGTCAATGTATTGACCCCCCCCCCCAAATACGAAAAAAAACCCCCCCCCCCCTGCTCATTAATGAC |
| ΔPyih_C | GTCATTAATGAGCAGGGGGGGGGGGGTTTTTTTTCGTATTTGGGGGGGGGGGTCAATACATTGACAGCTTTTTTTTTTTAAACATTTCG |
| ΔPyih_D | AGCCTGACGCGCCAGCGTAA |
| yihV1 | GCCCTCGAGCCGATAAATGCTATAACTG |
| yihV2 | GCCGGATCCCAAGCAATACGAACCATG |
a
PCR primers were purchased from Alpha DNA or University Core DNA and Protein Services (www.dnaservices.myweb.med.ucalgary.ca; University of Calgary).