TABLE 1.

Dependence of protein 1 phosphorylation with 100 μM [β-³²P]ATP on amounts of protein 2 and protein 3^a

Protein ratio (mol:mol)	% Pi incorporated into protein 1b
Protein 1:protein 2
1:0	0
1:1	11
1:2	33
1:5	50
Protein 1:protein 2:protein 3
1:5:1	100
1:5:2	100
1:5:5	100

^aTested in the presence of 1 M sucrose. The assay mixture (50 μl) contained 100 mM Tris-HCl, pH 8.5, 20 mM MgCl₂, 1 mM MnCl₂, 40 mM β-mercaptoethanol, 100 μM [β-³²P]ATP, 1 M sucrose, 7 μg of protein 1 (20 μM), and various amounts of protein 2 (20 to 100 μM) and protein 3 (20 to 100 μM). Incubation was carried out at 30°C for 30 min. For further details, see Materials and Methods.

^bOne hundred percent equals the degree of protein 1 phosphorylation under optimal conditions in the presence of protein 3.