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Purification of His6-tagged and untagged PrfA proteins from different Listeria strains. Coomassie blue staining of an SDS-PAGE (12%) gel loaded with 1 μg of the freshly purified His6-tagged PrfA proteins from L. seeligeri (PrfALs), L. ivanovii (PrfALi), the hybrid PrfALsm, and the untagged L. monocytogenes PrfALm and PrfA*Lm proteins. A protein molecular mass marker is shown to the right.
