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SPR analysis of nontagged PrfALm-PhlyLm PrfA box binding. The graph shows sensorgrams (gray) from a titration of coupled PhlyLm PrfA box DNA and nontagged PrfALm. The applied PrfA concentrations are listed to the right; corresponding response units (RU) are shown on the y axis. The black lines represent the fits of the association phase of each sensorgram. Resp. Diff., response difference.
