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Effects of increasing concentrations of RNA polymerase on formation of CI, CII, and CIII complexes. Electrophoretic mobility shift assays were performed with RNAP concentrations of 0.38, 0.75, 1.5, and 3 nM, with 0.23 μM PrfALm, 3.5 μM PrfALs (A), 6 nM PrfA*Lm, and 60 nM PrfALi (B) at 5 nM hly DNA promoter fragment of L. monocytogenes (PhlyLm). The data shown here represent the results of one of three independently performed experiments.
