FIG. 7.
Comparison of the hly and actA promoters of L. monocytogenes, L. ivanovii, and L. seeligeri in EMSA experiments. Specific binding affinity of 230 nM PrfALm, 12 nM PrfA*Lm, 3.5 μM PrfALs, and 60 nM PrfALi to the hly and actA promoter fragments of L. monocytogenes (PhlyLm and PactALm), L. ivanovii (PhlyLi and PactALi), and L. seeligeri (PhlyLs and PactALs). Quantification of the CI, CII, and CIII complexes, shown in the graphs to the right, was performed using ImageMaster (Amersham). The intensity of the band in lane 2 was again set to 1, and all other values are normalized to it. The data shown here represent the results of one of three independently performed experiments.