FIG. 8.
In vitro transcription assays with the hly and actA promoters of L. monocytogenes (PhlyLm and PactALm), L. ivanovii (i-Phly and i-PactA), and L. seeligeri (s-Phly and s-PactA). Results are from an in vitro transcription assay with increasing concentrations of the PrfA proteins PrfALm, PrfA*Lm, PrfALs, and PrfALi using 16 nM promoter template DNA and 1.3 nM (when using Phly) or 1.9 nM (when using PactA) RNA polymerase. The runoff transcripts were radioactively marked by adding [α-32P]CTP to the in vitro assay. The transcription-activating potentials of the different PrfA proteins compared to that of PrfALm are given in the graphs to the right. Values represent the relative ratios of the measured radioactivities and the molar concentrations of PrfA protein in the range of linear dependency. The data shown here represent the results of one of three independently performed experiments.