TABLE 2.

Surface plasmon resonance analysis: binding of different PrfA proteins to the Phly_Lm PrfA box^a

Protein	Rate constant		Equilibrium constant KD (M)
	ka (M−1 s−1)	kd (s−1)
Nontagged PrfALm	(6.1 ± 0.9) × 105	(2.7 ± 0.1) × 10−3	(4.6 ± 0.6) × 10−9
Nontagged PrfA*Lm	(∼1.9 ± 0.3) × 107	(1.6 ± 0.3) × 10−3	(∼8.2 ± 0.6) × 10−11
PrfALm	(2.2 ± 0.1) × 106	(1.3 ± 0.2) × 10−3	(6 ± 1) × 10−10
PrfALi	(3.7 ± 0.1) × 106	(1.2 ± 0.1) × 10−3	(3.6 ± 0.1) × 10−10

^aRate and equilibrium constants of nontagged PrfA_Lm, nontagged PrfA*_Lm, PrfA_Lm, and PrfA_Li binding to the Phly_Lm PrfA box. K_D is the ratio of the dissociation constant (k_d) to the association rate constant (k_a).