TABLE 1.

Validation of array data by real-time PCR^a

Gene identifier	Gene	Quantitative real-time RT-PCRb result (relative difference in expression change)			Expression difference of the hemB mutant analyses compared to the wild type using microarraysc
		hemB mutant	Complemented mutant	Wild type
SA0150	Capsular polysaccharide synthesis enzyme Cap5G	29.9	1.1	1.0	Sig. up-reg.
SA0232	l-Lactate dehydrogenase	21.1	1.0	1.2	Sig. up-reg.
SA0742	Fibrinogen-binding protein A	494.6	12.6	1.0	Sig. up-reg.
SA0922	Phosphoribosylpyrophosphate amidotransferase PurF	1.0	24.3	10.6	Sig. down-reg.
SA1141	Glycerol kinase	40.8	3.6	1.0	Sig. up-reg.
SA1142	Aerobic glycerol-3-phosphate dehydrogenase	128.0	1.0	2.4	Sig. up-reg.
SA2206	Immunoglobulin G-binding protein SBI	1.0	4.3	8.3	Sig. down-reg.
SA2424	Hypothetical protein, similar to transcription regulator Crp/Fnr family protein	163.1	1.0	1.3	Sig. up-reg.
SA2426	Arginine/ornithine antiporter	891.0	1.0	1.3	Sig. up-reg.
SA2428	Arginine deiminase	989.0	2.1	1.0	Sig. up-reg.

^aTranscription rates of selected genes for the S. aureus hemB mutant in comparison to the wild type and the complemented mutant for pooled time points. The relative differences in expression change were calculated using Gene Expression Analysis for iCycler iQ Real-Time PCR Detection System v1.10 (Bio-Rad). The lowest expression was set to 1.0. gyrA (SA0006) was used as a housekeeping gene for relative quantification.

^bRT-PCR, reverse transcription-PCR.

^cSee Table 2. Abbreviations: sig. up-reg., significantly up-regulated; sig down-reg., significantly down-regulated.