Skip to main content
. 2006 Sep 15;188(22):7905–7913. doi: 10.1128/JB.00997-06

FIG. 4.

FIG. 4.

Detection of BclA and other glycoproteins in washed exosporium following SDS-PAGE on 10 to 20% gradient Tris-glycine gels. (A) Western blot of proteins from washed exosporium probed with CR1, an anti-BclA monoclonal antibody. (B) Total glycoprotein profile of the same exosporium samples. (C) Proteins concentrated from spore culture supernatants, again probed with CR1 antibody to detect BclA. Lane 1, B. cereus ATCC 10876 (wild type); lane 2, AM1652 (ΔexsY); lane 3, AM1653 (ΔcotY). In panel C; lane 4 shows AM1654 (ΔexsY ΔcotY).