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Detection of ExsY and CotY in exosporium fractions. Western blots are shown with primary polyclonal antibodies as follows: (A) EY-GST (raised against purified ExsY/GST, which detects both ExsY and CotY), (B) EY-N (raised against an ExsY-specific peptide), and (C) CY-N (raised against a CotY-specific peptide). Lane 1, B. cereus ATCC 10876 (wild type); lane 2, AM1652 (ΔexsY); lane 3, AM1653 (ΔcotY). ExsY and CotY monomers migrate at 16 kDa.
