TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory stock |
| TG1 | supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK− mK−) [F′ traD36 proAB lacIqlacZΔM15] | Laboratory stock |
| HB101 | supE44 hsdS20(rB− mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1 | Laboratory stock |
| BL21(DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3) | Novagen |
| B. cereus | ||
| ATCC 10876 | trp-1 Strr | 7 |
| AM1652 | trp-1 StrrexsY::spc | This study |
| AM1653 | trp-1 StrrcotY::erm | This study |
| AM1654 | trp-1 StrrexsY::spc cotY::erm | This study |
| SJT003 | trp-1 StrrexsY::Tn917-LTV1 | This study |
| Plasmids | ||
| pUC19 | Ampr | 35 |
| pAT113 | Eryr Kanr | 34 |
| pFX113 | Spcr Kanr | 3 |
| pUC1318Erm | Eryr Ampr | 33 |
| pUC1318Spc | Spcr Ampr | 33 |
| pEY-Spc | Spcr Eryr KanrexsY::spc | This study |
| pCY-Ery | Spcr Eryr KanrcotY::erm | This study |