FIG. 1.

Transcriptional analysis of the sae operon. (A) Northern blot analysis of the sae operon. RNA was isolated from S. aureus COL and S. aureus Newman grown in TSB medium at 37°C. The membrane was hybridized with digoxigenin-labeled RNA probes specific for saeR. Relevant transcripts are indicated by arrows. (B) Methylene blue-stained blots are shown as indication for equal RNA loading. (C) Growth curve of S. aureus COL and Newman. The sampling points are indicated in the growth curve by arrows and optical density numbers. (D) Schematic representations of the gene loci based on the sequence of S. aureus COL and the transcriptional organization of the predicted operons. The dotted line represents the RNA probe used in the experiments.