Fig. 2.

Matched normal and neoplastic tissues analyzed in the RMC assay. Hematoxylin/eosin-stained sections of the paired normal (Left) and tumor (Right) tissues listed in Table 1 are shown. The tissues are normal squamous vaginal epithelium and high-grade papillary serous ovarian carcinoma with psammoma bodies; normal renal cortex and dedifferentiated sclerosing perirenal liposarcoma; normal colonic mucosa and invasive colonic adenocarcinoma; renal cortex with lymphocytic inflammation and malignant renal epithelioid angiomyolipoma; and normal skeletal muscle and high-grade malignant fibrous histiocytoma pleomorphic sarcoma. Immunohistochemical analysis of MLH1 and MSH2 proteins involved in DNA mismatch repair showed the colonic adenocarcinoma to lack MLH1 expression and to have normal expression of MSH2. These results suggest that the tumor is defective in mismatch repair. The mutation frequencies measured in the RMC assay are indicated below each section.