Figure 6.

(A) Homo-FRET experiments on hybrid i-motif of 1:1 TMR-p(C₅T) : d(TC₅) at 25 μM total strand concentration. The transfer efficiency was measured with respect to single-stranded 25 μM TMR-p(C₅T) only (black). Inset: thermal denaturation of fluorescently labeled hybrid i-motif. Fluorescence intensity at 580 nm was followed at a function of temperature. (B) Analogous Homo-FRET measurements on hybrid i-motif with 3′ labeled DNA strands, 3′-TMR-d(TC₅) : p(C₅T). Transfer efficiency was measured with respect to single-stranded 25 μM 3′-TMR-d(TC₅) only. Inset: UV melting of fluorescently labeled hybrid i-motif followed at 295 nm as a function of temperature.