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Subcellular localization of DGCR8 and its deletion mutants. HeLa cells were transiently transfected with V5-tagged constructs, including WT DGCR8, and the DG276–773, DG484–773, DG1–483, DG1–614 and DG1–692 mutants. The left panels show DGCR8 protein signals visualized with rabbit anti-V5 serum (1:500) and Alexa Flour 594 goat anti-rabbit IgG (1:400). The middle panels show DAPI staining of the nucleus and the right panels display an overlay of these two signals.
