Figure 4.

Gel mobility shift assay for the protein transfer between ssDNA molecules. A constant amount of Dmc1 (10 µM) was incubated with φX174 circular ssDNA (20 µM) in the absence (lanes 2–6) or presence (lanes 7–11) of Rad54B (200 nM), followed by an incubation with increasing amounts of φX174 ssDNA fragments (0, 25, 50, 100 and 200 µM in lanes 2–6 and lanes 7–11, respectively) at 37°C for 2 h. In lane 1, φX174 circular ssDNA was incubated in buffer without any recombinant proteins.