Table 2.
Structural statisticsa
| 27 nt | 8 nt loop | |
|---|---|---|
| Violations of experimental restraints | ||
| Mean number of NOE violations >0.1 Å | 11.8 ± 1.3 | 5.0 ± 1.4 |
| Maximum NOE violation (Å) | 0.38 | 0.37 |
| Mean number of torsion angle violations >2° | 0.5 ± 0.5 | 0 |
| Maximum torsion angle violation (°) | 3.7 | 1.9 |
| The r.m.s.d. of RDC violation (Hz) | 1.61 ± 0.16 | |
| Alignment tensor statistics | ||
| Axial component, Da (Hz)b | −26.9 ± 1.8 | |
| Rhombicityb | 0.17 ± 0.06 | |
| Axial component, Da (Hz)c | −24.7 ± 1.1 | |
| Rhombicityc | 0.19 ± 0.04 | |
| The r.m.s.d. values from ideal covalent geometry | ||
| Bond lengths (Å) | 0.012 ± 0.000 | 0.011 ± 0.001 |
| Bond angles (°) | 2.81 ± 0.07 | 2.74 ± 0.25 |
| Atomic r.m.s.d. from average structure | ||
| Stem I (27 nt) (Å)d | 0.64 | |
| Stem II (27 nt) (Å)d | 0.79 | |
| Residues 10, 11, 15 and 17 (Å) | 1.31 | |
| Overall (Å) | 1.92 | 2.71 |
aFor the complete 27 nt molecule, 20 structures selected in step B3 are included in the analysis; for the 8 nt loop, 12 structures selected in step A2 are included.
bCalculated by SVD using PALES.
cCalculated using the gyration tensor method.
dFor the 27 nt molecule: stem I = residues 1–4 and 24–27; stem II = residues 5–10 and 17–22.