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. 2006 Aug 31;34(16):4458–4466. doi: 10.1093/nar/gkl587

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Synthesis of oligonucleotides containing N-3-chloroethylthymidine. Reagents and conditions: (a) Cs₂CO₃, BrCH₂CH₂Cl, DMF, 86%. (b) TBAF, THF, 89%. (c) ClP(N(iPr)₂)(OEC), (iPr)₂NEt, CH₂Cl₂, 90%. (d) Solid-phase DNA synthesis and deprotection.