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. 2006 Aug 31;34(16):4495–4505. doi: 10.1093/nar/gkl614

Figure 4.

Figure 4

The repeat orientation relative to the direction of replication influences CAG·CTG contractions in SVG-A cells. (A) Complementary CAG and CTG repeats can differentially affect TNR instability based on the orientation of the repeat relative to the direction of replication. In the left panel, CAG repeats reside on the lagging strand template. In the middle panel, the reverse orientation places the CTG repeats on the lagging template strand. On the right, switching the position of the SV40 ori reverses the direction of replication and swaps the assignment of leading and lagging strand. The direction of replication was deduced from the position of the S40 ori relative to the TNR. (B) Contraction frequency (%) is plotted as given in Table 1 (column 9) for the indicated starting alleles. Error bars indicate ±1 SEM. (C) Contraction frequency (%) is plotted versus CTG repeat length. All values in (B and C) represent the corrected contraction frequency in SVG-A cells.