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. 2006 Aug 31;34(16):4495–4505. doi: 10.1093/nar/gkl614

Figure 5.

Figure 5

Influence of interruptions on CTG repeat contractions in SVG-A cells. (A) Corrected contraction frequencies (Table 1, entries 9L and 9M) are plotted for starting tracts of (CTG)6(ATG)2(CTG)25 (filled bar) and for (CTG)33 (unfilled bar). Error bars indicate ±1 SEM. (B) Mutation spectra for (CTG)6(ATG)2(CTG)25 (filled bars) versus (CTG)33 (unfilled bars). The number of observed events is plotted on the ordinate versus the size of the contraction (as determined by PCR) on the abscissa. (C) Representative gel showing results of the SfaNI-resistance assay. PCR products were analyzed on 6% denaturing polyacrylamide gels prior to (odd number lanes) or following (even number lanes) treatment with SfaNI, as described in Materials and Methods. Lanes 1–4 show results with PCR products from control 33-repeat plasmids that are interrupted (lanes 1 and 2) or perfect (lanes 3 and 4). The asterisk indicates the starting position of the 33-repeat uncut and SfaNI-resistant perfect repeat tracts. Lanes 5–10 show results with PCR products from contractions of the (CTG)6(ATG)2(CTG)25 allele in SVG-A cells. The letters a–c indicate the position of the uncut samples in lanes 5, 7 and 9, respectively. The letters below the gel image indicate the assignment of SfaNI sensitivity (S) or resistance (R). To become resistant, both ATG interruptions must be lost.