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. 2006 Aug 31;34(16):4527–4536. doi: 10.1093/nar/gkl628

Figure 1.

Figure 1

Genomically integrated cassettes for constitutive or Tet-inducible expression of shRNA/Plk1 or shRNA/Plk1MM. (A) Different promoter derivatives for constitutive or Tet-inducible expression of shRNA/Plk1 or shRNA/Plk1MM were stably integrated into TetR-expressing HeLa cells followed by isolation of single cell clones: wild-type H1 promoter for constitutive expression of mismatch shRNA, US-type promoter containing TetO upstream of the TATA box; DS-type promoter containing TetO downstream of the TATA box and the US/DS-type promoter containing TetO upstream and TetO downstream of the TATA box. TetO, tetracycline operon; TATA, TATA box. (B) Schematic representation of promoter function in absence (−Dox) and presence of doxycycline (+Dox).