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. 2006 Aug 31;34(16):4527–4536. doi: 10.1093/nar/gkl628

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Proliferative activity of inducible cell clones expressing shRNA/Plk1MM or shRNA/Plk1. HeLa cell clones containing stably integrated H1 promoter derivatives were induced for the expression of shRNA/Plk1MM or shRNA/Plk1MM by addition of 5 μg/ml Dox to the culture medium. (A) Proliferative activity of cell clones containing promoter of the US-type for the expression of shRNA/Plk1MM or shRNA/Plk1; (B) proliferative activity of cell clones containing promoter of the DS-type for the expression of shRNA/Plk1MM or shRNA/Plk1; (C) proliferative activity of cell clones containing promoter of the US/DS-type for the expression of shRNA/Plk1MM or shRNA/Plk1. Values are shown in percent compared to wild-type HeLa cells. Significant growth inhibitions (P < 0.05) are indicated with an asterisk.