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. 2006 Aug 31;34(16):4527–4536. doi: 10.1093/nar/gkl628

Figure 5.

Figure 5

Tumor growth in nude mice after Dox-induced expression of shRNA/Plk1MM or shRNA/Plk1. Mice were inoculated with 1 × 107 HeLa cell clones in each flank HeLa clones for inducible expression of shRNA/Plk1MM were inoculated on the left flank whereas clones for inducible expression of shRNA/Plk1 were inoculated on the right flank. (A) Relative tumor volumes of xenografted US-type HeLa tumors for inducible expression of shRNA/Plk1MM or shRNA/Plk1 either after induction with Dox or in the absence of Dox starting 23 days after inoculation. Significant inhibition of tumor growth (P < 0.05) is indicated with an asterisk. (B) Northern blot analysis of Plk1 mRNA under the influence of shRNA/Plk1MM or shRNA/Plk1 expression. Plk1 mRNA expression in US-type promoter-derived HeLa tumors of mice without Dox-treatment (lanes 1 and 3) in comparison to tumors of mice treated with Dox (lanes 2 and 4). For standardization ethidiumbromide staining of 28S rRNA was chosen. Significant reduction (P < 0.05) is indicated with an asterisk.