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. 2006 Aug 31;34(16):4537–4545. doi: 10.1093/nar/gkl634

Figure 1.

Figure 1

Construction of plastid transformation vectors for disruption of rps18. (A) Physical maps of the rps18 region in the tobacco plastid genome [ptDNA; Ref. (15)]. Genes above the lines are transcribed from the left to the right, genes below the lines are transcribed in the opposite direction. (B) Map of transformation vector pΔrps18A. Note that the spectinomycin resistance gene aadA is driven by the endogenous rps18 promoter. (C) Map of transformation vector pΔrps18B. In this vector, the rRNA operon-derived chimeric Prrn promoter (16) drives the selectable marker gene aadA. Restriction sites used for cloning, RFLP analysis and/or generation of hybridization probes are indicated. Sites lost owing to ligation to heterologous ends are shown in parentheses. The hybridization probe (NdeI/SpeI fragment) is indicated and the expected sizes of hybridizing bands in the two RFLP analyses (Figure 2) is shown below each map. The chloroplast targeting fragment in transformation vectors pΔrps18A and pΔrps18B is marked by dashed lines (NdeI/EcoRI fragment cloned into pUC18).