Table 1.
Segregation of the aadA antibiotic resistance gene disrupting rps18 in the T1 generation
| Plant line | Resistant seedlings | ||
|---|---|---|---|
| Test 1 | Test 2 | Total | |
| Wild type | 0 (0.0%) | 0 (0.0%) | 0 (0.0%) |
| Nt-Δrps18A-13 | 6 (0.4%) | 13 (0.9%) | 19 (0.6%) |
| Nt-Δrps18A-14 | 22 (1.5%) | 29 (1.9%) | 51 (1.7%) |
| Nt-Δrps18A-18 | 20 (1.3%) | 12 (0.8%) | 32 (1.1%) |
| Nt-Δrps18B-2 | 17 (1.1%) | 16 (1.1%) | 33 (1.1%) |
| Nt-Δrps18B-15 | 30 (2%) | 39 (2.6%) | 69 (2.3%) |
| Nt-Δrps18B-27 | 7 (0.5%) | 2 (0.1%) | 9 (0.3%) |
Approximately 3000 seeds per line (obtained from self-fertilized plants) were sown on spectinomycin-containing medium and assayed for spectinomycin resistance in two independent experiments for each line.