Table 2.
Amplified and assembled DNA sequence data
| Oligo | #Clones | WT | 1MM | 2MM | >2MM |
|---|---|---|---|---|---|
| A | 25 | 24% | 64% | 8% | 4% |
| B | 20 | 35% | 35% | 25% | 5% |
| Product (AB) | 18 | 0% | 17% | 33% | 50% |
| 21,22 | 42 | 10% | 7% | 16% | 67% |
| 23,24 | 45 | 44% | 11% | 11% | 33% |
| Product (21–24) | 17 | 6% | 23% | 23% | 47% |
Sequences containing no mutations are denoted as wild-type(WT); single mutations as one mismatch(1MM); two mutations as two mismatch (2MM); two mutations or more as (>2MM).