Skip to main content
. 2006 Sep 29;34(18):5270–5279. doi: 10.1093/nar/gkl489

Figure 5.

Figure 5

DNA binding of MutSCF and MutSCF/D835R variants using EMSA. (A) MutSCF or MutSCF/D835R binding to 42 bp G/T heteroduplex DNA, respectively, was performed in the presence of 0.5 mM ADP. MutS concentrations (monomer equivalents) were 1, 5, 12.5, 25, 50, 65, 80, 100, 300 nM (lanes 2–10 and 11–19). Arrows C1, C2 and C3 indicate different MutS–DNA complexes. Note that complex C1 is not observed for the tetrameric MutSCF meanwhile complex C3 is not observed for the dimeric MutSCF/D835R. (B) Quantitative analysis of DNA binding for MutSCF (circles) and MutSCF/D835R (triangles). Fits to a sigmoidal Hill function (see Materials and Methods) yielded apparent K1/2 = 42 ± 2 nM for MutSCF (Hill coefficient n = 3.6 ± 0.3) and K1/2 = 45 ± 3 nM for MutSCF/D835R (Hill coefficient n = 2 ± 0.2).