Table 3.
Molecular masses and oligomerization state of MutS variants
| Protein | Calculated mass (kDa) | Size-exclusion chromatography (Mapp/kDa) | AUC (M/kDa) | Oligomeric state | Reference |
|---|---|---|---|---|---|
| MutSwt | 381 | ≈580a | 360 ± 20 | Dimer/Tetramer | (7,8,31) |
| MutSwt | 390 | ≈450a | N.D. | Dimer/Tetramer | this studyd |
| MutSCF | 390 | ≈450a | N.D. | Dimer/Tetramer | this studyd |
| MutS800 | 180 | N.D. | 220 ± 12 | Dimer/Monomer | (7,8) |
| MutS800CF | 183 | 125/175b | N.D. | Dimer/Monomer | this studyd |
| MutSCF/D835R | 190 | ≈180c | N.D. | Dimer | this studyd |
aConcentration dependent in a range of 0.5–10 μM.
bIn the absence or presence of ADP.
cConcentration independent in a range of 0.5–17 μM.
dN-terminal His6-tagged proteins.