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. 2006 Sep 29;34(18):5300–5311. doi: 10.1093/nar/gkl565

Figure 2.

Figure 2

Detail schematic representation of filtering subtask of ProSeq identification Task(I). For each ProSeq, primer regions were masked as N (ambiguous) calls, then UniRate, was calculated from the HybSeq. For ProSeqs, which passed the UniRate requirement, a revised sliding window algorithm attempted to grow a SubSeq that could be used as a query to BLAST. The identity (start location in the ProSeq and length) of a successfully grown SubSeq was placed in a file for batch querying via BLAST. VARI = [(‘SubSeq length’ − 30) * 0.2857 + 70]. Detailed SubSeqs requirement is described in Supplementary Data.