Table 4.
Organism identification and algorithm decisions from Vaccinia sample on Variola Major virus ProSeqs for SubSeq, ProSeq identification Task(I), ProSeq grouping Task(II) and pathogen determination Task(III)
| CFU | ProSeq | Unique calls (%) | # SubSeq | SubSeq organism identification and Uniqueness, Bit score | Task I | Task II | Task III |
|---|---|---|---|---|---|---|---|
| 5 × 107 | CRMB | 77.90 | 2 | Orth.TA 156(H1), Vacc.TU 153(H2) | Vacc., TU | Vacc., TU | Detected |
| HA | 29.40 | 1 | Orth.TA 60.2(H3) | Orth., TA | Orth., TA | Vaccinia | |
| 5 × 107 | CRMB | 79.80 | 2 | Orth.TA 164(H1), Vacc.TU 115(H2) | Vacc., TU | Vacc., TU | Detected |
| HA | 25.70 | 1 | Orth.TA 66.6(H3) | Orth., TA | Orth., TA | Vaccinia | |
| 1.6 × 107 | CRMB | 79.40 | 2 | Orth.TA 161(H1), Vacc.TU 114(H2) | Vacc., TU | Vacc., TU | Detected |
| HA | 14.80 | 0 | Null | Null | Vaccinia | ||
| 1.6 × 107 | CRMB | 77.50 | 2 | Orth.TA 153(H1),109(H4) | Orth., TA | Orth., TA | Detected |
| HA | 24.50 | 0 | Null | Null | Orthopox | ||
| 1.6 × 107 | CRMB | 76.80 | 2 | Orth.TA 155(H1), Vacc.TU 112(H2) | Vacc., TU | Vacc., TU | Detected |
| HA | 21.60 | 0 | Null | Null | Vaccinia | ||
| 1.6 × 107 | CRMB | 74.50 | 2 | Orth.TA 152(H1), 106(H5) | Orth., TA | Orth., TA | Detected |
| HA | 17.30 | 0 | Null | Null | Orthopox | ||
| 5 × 106 | CRMB | 77.90 | 2 | Orth.TA 155(H1), Vacc.TU 112(H2) | Vacc., TU | Vacc., TU | Detected |
| HA | 25.70 | 0 | Null | Null | Vaccinia | ||
| 5 × 106 | CRMB | 78.30 | 2 | Orth.TA 153(H1), 115(H5) | Orth., TA | Orth., TA | Detected |
| HA | 22.00 | 0 | Null | Null | Orthopox | ||
| 5 × 106 | CRMB | 73.00 | 2 | Orth.TA 150(H1), Vacc.TU 99.9(H2) | Vacc., TU | Vacc., TU | Detected |
| HA | 13.00 | 0 | Null | Null | Vaccinia | ||
| 5 × 106 | CRMB | 73.40 | 2 | Orth.TA 153(H1), 115(H5) | Orth., TA | Orth., TA | Detected |
| HA | 7.80 | 0 | Null | Null | Orthopox | ||
| 1.6 × 106 | CRMB | 75.30 | 2 | Orth.TA 158(H1), 107(H5) | Orth., TA | Orth., TA | Detected |
| HA | 8.60 | 0 | Null | Null | Orthopox | ||
| 1.6 × 106 | CRMB | 49.80 | 2 | Orth.TA 60(H1), Vacc.TU 90.3(H2) | Vacc., TU | Vacc., TU | Detected |
| HA | 6.60 | 0 | Null | Null | Vaccinia | ||
| 1.6 × 106 | CRMB | 65.50 | 2 | Orth.TA 136(H1), 91.9(H5) | Orth., TA | Orth., TA | Detected |
| HA | 10.00 | 0 | Null | Null | Orthopox | ||
| 1.6 × 106 | CRMB | 62.90 | 2 | Orth.TA 126(H1), 87.2(H5) | Orth., TA | Orth., TA | Detected |
| HA | 8.20 | 0 | Null | Null | Orthopox | ||
| 5 × 105 | CRMB | 58.40 | 2 | Orth.TA 110(H1), 90.3(H5) | Orth., TA | Orth., TA | Detected |
| HA | 9.00 | 0 | Null | Null | Orthopox | ||
| 5 × 105 | CRMB | 56.20 | 2 | Orth.TA 77.7(H6), 96.7(H5) | Orth., TA | Orth., TA | Detected |
| HA | 8.00 | 0 | Null | Null | Orthopox | ||
| 5 × 105 | CRMB | 49.00 | 1 | Orth.TA 87.2(H5) | Orth., TA | Orth., TA | Detected |
| HA | 9.30 | 0 | Null | Null | Orthopox | ||
| 5 × 105 | CRMB | 44.60 | 1 | Orth.TA 90.3(H5) | Orth., TA | Orth., TA | Detected |
| HA | 7.80 | 0 | Null | Null | Orthopox |
Vacc., Vaccinia; Orth., Orthopox. (H1) Rabbitpox, Buffalopox, Cowpox, Vaccinia, Callithrix jacchus, Taterapox. (H2) Vaccinia. (H3) Vaccinia, Variola (Major and Minor), Cantagalo, Ectromelia, Elephantpox, Aracatuba, Cowpox, Taterapox. (H4) H2 and Cowpox. (H5) H4 and Camelpox. (H6) H1 and Variola, Variola Major, Variola Minor.