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. 2006 Sep 29;34(18):5312–5324. doi: 10.1093/nar/gkl598

Figure 6.

Figure 6

(A) Northern blots of CAT mRNA provided with successive deletions of the COX V, COX VI or COX IX intergenic regions. In each case the rRNA region of the ethidium stained gel is provided to demonstrate relative loading. (B) Northern blots of CAT mRNA derived from cell lines in which the CAT gene is provided with the COX IX intergenic region (9IR) or a deletion (COX IX Δ4, ‘9Δ4’) which results in altered mRNA abundance. All cell lines were exposed to actinomycin D at t = 0 and RNA isolated at time points thereafter in order to follow transcript decay. The relative abundance of CAT mRNA is denoted beneath each lane. (C) Northern blot of CAT mRNA derived from cells transfected with constructs comprising either the intact intergenic region for COX V or a deleted derivative (COX VΔ4; ‘5Δ4’). In each case cells were either untreated (−) or treated (+) with cycloheximide, with mRNA being isolated 4 h after the addition of drug. CAT mRNA abundance is denoted beneath each lane.