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. 2006 Sep 22;34(18):5133–5144. doi: 10.1093/nar/gkl610

Figure 4.

Figure 4

The expanded H8/H6–H1′ region with assignments of the non-exchangeable 2D-NOESY spectrum of bcl2Mid.The sequential assignment pathway is shown. Missing connectivities are labeled with asterisks. The H8–H1′ NOEs of the nucleotides with syn configuration are labeled by residue names, while the H5–H6 NOEs of cytosines are also labeled for reference. The H8–H1′ NOE crosspeak of G21 has a large integration value; however, the H8 and H1′ of G21 are much broader than those of other guanines and give rise to a much broader H8–H1′ NOE crosspeak. The characteristic G(i)H8/G(i+1)H1′ NOEs for the syn G(i)s are labeled as a–b for G1–G2 and G7–G8. The NOE between C6H1′ and G19H8 is labeled as c. Conditions: 25°C, 20 mM K-phosphate, 40 mM KCl, pH 7.0, 1.5 mM DNA.