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(A) 1D proton-decoupled 31P NMR spectrum of bcl2Mid with phosphorus assignments. (B) 2D heteronuclear 31P–1H Correlation Spectroscopy (COSY) of bcl2Mid with peak assignments. The NnP-N(n-1)H3′ crosspeaks are labeled as Nn-N(n-1). The spectra were referenced to external H3PO4. Conditions: 25°C, 20 mM K-phosphate, 40 mM KCl, pH 7.0, 1.5 mM DNA.
