The basic NLS2 mediates nuclear import of MoKA. (A) At the top is a schematic representation of the MoKA protein structure showing the relative locations of the three putative basic NLSs identified by sequence homology. Below are illustrative confocal images of indirect immunofluorescence of COS7 cells transiently expressing NLSs1–3-containing peptides fused to PK-myc (NLS1-PK, NLS2-PK, and NLS3-PK) and with the NLS2-containing peptide carrying the K901E/K903E mutation fused to PK-myc (NLS2mut-PK). The bar graphs on the lower right summarize the individual N/C ratios of PK, NLS1-PK, NLS2-PK, NLS3-PK, and NLS2mut-PK fusion products. (B) Images of COS7 cells transiently expressing wild-type MoKA-GFP and a mutant version of it harboring the K901E/K903E mutations (MoKANLS2mut-GFP). Bar graphs on the right summarize the individual N/C ratios of MoKA-GFP and MoKANLS2mut-GFP fusion products.